20 Feb 2017
by Madolyn Bowman Rogers
This article appears by special arrangement with Alzforum. See original article with additional links/commentary.
Researchers routinely use FDG PET scans, which measure brain glucose consumption, as markers of neural activity. Now, a new paper challenges the idea that this signal solely represents neuronal function. In the January 30 Nature Neuroscience, researchers led by Pedro Rosa-Neto at McGill University, Montreal, report that stimulating glucose uptake by astrocytes in the living rat brain enhanced the FDG PET signal. “This has implications for understanding what FDG PET means as an outcome measure in clinical trials. It may reflect intervention effects on astrocytes as well as neurons,” Rosa-Neto told Alzforum.
Others noted that the authors did not determine whether astrocytes contribute to the FDG PET signals in rats under normal physiological conditions. “The idea that a significant portion of the FDG PET signal can be driven by astrocytes needs confirmation from other labs and by other methods. I consider it an intriguing, provocative hypothesis worthy of further investigation,” William Klunk at the University of Pittsburgh wrote to Alzforum.
[18F]FDG, short for fluorodeoxyglucose, is a radiolabeled sugar analog that allows researchers to track glucose uptake by brain cells. While brain glucose consumption has been assumed to reflect primarily neuronal activity, other researchers have pointed out that activated neurons release glutamate, which stimulates astrocytes to take up glucose as well (see Sokoloff 1993; Pellerin and Magistretti, 1994; Pellerin and Magistretti, 2012). Several groups have noted that this process could in theory affect the FDG PET signal (see Magistretti and Pellerin, 1996; Nehlig and Coles, 2007; Figley and Stroman, 2011).
However, no one had shown that astrocytes contribute to FDG PET in the living brain. To do this, first author Eduardo Zimmer used ceftriaxone. Besides fighting bacteria, this antibiotic drug happens to activate the astrocytic glutamate transporter GLT-1, which in turn triggers glucose uptake (see Lee et al., 2008). The authors injected ceftriaxone into the tail veins of adult rats, then injected [18F]FDG 30 minutes later and scanned the animals 40 minutes after that. Compared to rats injected with saline, FDG uptake values in rats on ceftriaxone ran about 15 percent higher in several brain regions, including hippocampus, striatum, thalamus, and temporoparietal cortex. The changes were statistically significant, with the prefrontal cortex notching the most notable boost, of about 20 percent (see image above).
Several lines of evidence reinforced the idea that astrocyte activation drove the enhanced signal. The most affected regions are reported to express the highest levels of GLT-1, according to the Allen Brain Atlas. On the other hand, the FDG PET signal barely budged in the cerebellum, which is reported to express little GLT-1. Also, blood flowed at normal rates through the cerebrum in treated rats. Because active neurons release glutamate, which causes blood vessels to dilate, this to the authors indicated no difference in neuronal activity in treated rats.
The finding should stimulate more research, commenters suggested. “This is a great example of the interaction between the cell types, and demonstrates that using imaging can help elucidate those interactions,” Arthur Toga at the University of Southern California, Los Angeles, wrote to Alzforum.
What does this mean for AD studies? Brain glucose use goes down in people with prodromal AD, and is low in adult carriers of the ApoE4 allele regardless of their cognitive health. If astrocytes do play a role in FDG PET in the resting-state brain, that could tweak the interpretation of scans in past studies, but would not invalidate that research, commenters agreed.
“We know that glucose hypometabolism in AD at least partially reflects neuronal and synaptic loss and dysfunction, but there are other factors at work, such as disconnection of distant brain regions,” Gaël Chételat at INSERM-EPHE-University of Caen, France, told Alzforum. “This [paper] provides additional evidence that hypometabolism reflects a broader mechanism than just neuronal and synaptic loss.” For his part, Klunk noted that the new data creates a puzzle, because astrogliosis develops around plaques in AD brain. If activated astrocytes drive the FDG PET signal, why would it go down in AD and not up?
In future work, Rosa-Neto will try to determine the precise role of astrocytes in the FDG PET signal by combining PET scans with fMRI and electrophysiology. He will also study how therapeutic interventions affect aerobic metabolism and astrocyte-neuron interactions.