Schizophrenia Research Forum - A Catalyst for Creative Thinking

What Role BDNF?—A Question of Maturity

Article appears by special arrangement with Alzheimer Research Forum. See original article with additional links/commentary.

1 August 2005. In life, you wouldn’t trust a child to do an adult’s job. In the brain, the same might be true. While the mature form of brain-derived neurotrophic factor (BDNF) may stimulate long-term potentiation (LTP), or the strengthening of synaptic connections that is required for learning and memory, the immature, proBDNF not only fails in this regard, it does exactly the opposite, stimulating long-term depression (LTD). So concludes an article in last week’s Nature Neuroscience online.

Bai Lu, Cornell University, New York, and colleagues there and at the National Institute of Child Health and Human Development, Bethesda, Maryland, realized the rebellious nature of the immature neurotrophic factor when studying the effect of the neurotrophin receptor, p75NTR. This cell surface receptor binds to a variety of ligands and is known to promote apoptosis, or programmed cell death. However, ablating the gene for p75NTR in mice does little to prevent neuronal cell death, and in fact leads to sensory deficits, suggesting the receptor has other roles besides triggering apoptosis (see Lee et al., 1992).

To pinpoint what else p75NTR might get up to in the brain, first author Newton Woo and colleagues characterized the electrophysiological properties of hippocampal slices from p75NTR-negative mice. Woo found that while basic neurotransmission was normal in CA1 neurons, LTD elicited by transmission through the N-methyl-D-aspartate glutamate receptor was significantly compromised. In p75NTR-/- neurons, low frequency stimulation did stimulate LTD at first, but within 60 minutes transmission was fully back to normal, whereas LTD persists in p75NTR+/+ neurons for many hours.

The high-affinity ligand for p75, proBDNF, came in for scrutiny next. When Woo preincubated hippocampal slices with the rookie neurotrophin, then stimulated LTD, he found there was a small (about 17 percent) yet significant enhancement of the depression. However, in p75NTR-/- hippocampal slices, proBDNF had no effect on LTD, confirming the involvement of the neurotrophin receptor. ProBDNF also had no effect in the presence of ifenprodil, an inhibitor of the NR2B subunit of the NMDA receptor, suggesting that the proBDNF/p75NTR interaction acts through this subunit, which is known to be involved in LTD. In fact, using immunohistochemistry measurements, Woo and colleagues found that p75NTR is colocalized in the hippocampus with postsynaptic density 95 (PSD95), a protein normally found in synapses. This localization puts the neurotrophin receptor in close proximity to NR2B. Furthermore, when the authors treated hippocampal slices with proBDNF, they observed about a 50 percent increase in NMDA currents, but only in p75NTR+/+ animals.

“These results provide strong support for the idea that endogenously secreted proBDNF, by acting on p75NTR, promotes NMDA-dependent LTD by enhancing the expression of NR2B at CA1 synapses,” write the authors. The results also support the growing realization that immature neurotrophins try to resist the actions of their mature counterparts. Thus, while BDNF and proBDNF stimulate LTP and LTD, respectively, neurotrophic growth factor (NGF) and proNGF protect or stimulate neuronal apoptosis, respectively, through their actions on p75NTR and tyrosine receptor kinase A (TrkA).

Of course, various neurotrophic factors have been tested in laboratory animals and in clinical trials for their abilities to protect against neurodegeneration. While a phase I clinical trial of glial-derived neurotrophic factor in patients with Parkinson disease has recently been halted (see Alzheimer Research Forum related news story), a small trial of NGF in AD patients has produced some promising results (see Alzheimer Research Forum related news story). As for BDNF, numerous studies have shown that levels of both forms of the protein are lowered in various parts of the AD brain (see Alzheimer Research Forum related news story). Now, these latest findings suggest that the ratio of pro- and mature BDNF might be a key factor in regulating the balance between LTP and LTD. For this reason, understanding what prompts the rookie proBDNF to mature may be crucial.—Tom Fagan.

Reference:
Woo NH, Teng HK, Siao C-J, Chiaruttini C, Pang PT, Milner TA, Hempstead BL, Lu B. Activation of p75NTR by proBDNF facilitates hippocampal long-term depression. Nat Neurosci. 2005 Aug;8(8):1069-77. Epub 2005 Jul 17. Abstract

Comments on Related News


Related News: BDNF In the Nucleus Accumbens—Too Much of a Good Thing?

Comment by:  NN Kudryavtseva
Submitted 23 February 2006
Posted 23 February 2006

Berton and colleagues show very impressive data of molecular studies demonstrating numerous changes of gene expression in brain under repeated social defeats. However, the behavioral or pharmacological data that the authors use to support the development of depression in socially defeated mice may be interpreted otherwise.

The authors used decreases in the level of social communication (they called it avoidance-approach behavior) in defeated losers as parameters of depression. We repeatedly noted in our experiments on the social model of depression induced by social confrontations in mice of the C57BL/6J strain (Kudryavtseva et al., 1991) that even one or two social defeats lead to a decrease of communication in mice. Thus, avoidance behavior cannot be used as a specific parameter of depression; rather, it may represent anxiety. However, our experiments demonstrated that longer experience of defeats over 20-30 days (but not 10 days, as used by Berton et al.) in male mice produces development of a depression-like state (anxious depression): similarities of symptoms, etiological factors (social unavoidable emotional stress, permanent anxiety), sensitivity to chronic antidepressants and anxiolytics (imipramine, tianeptine, citalopram, fluoxetine, buspirone, etc.), as well as brain neurochemistry changes (serotonergic and dopaminergic systems) (Kudryavtseva et al., 1991; for reviews see Kudryavtseva, Avgustinovich, 1998; Avgustinovich et al., 2004).

In our molecular studies, we also demonstrated changes of gene expression in the brains of male mice after daily agonistic interactions. Three experimental groups were compared: the losers with repeated experience of social defeats; winners with repeated aggression accompanied by social victories; and controls (very important—the same strain). In has been shown that MAOA and SERT mRNA levels in the raphe nuclei of the losers were higher than in the controls and winners. TH and DAT gene expression in the ventral tegmental area was higher and κ opioid receptor gene expression was lower in the winners in comparison with the losers and controls (see Filipenko et al., 2001; 2002; Goloshchapov et al., 2005; reviewed in Kudryavtseva et al., 2004). Thus, there are different specific changes in gene expression in different brain areas in male mice with opposite social behaviors—winners and losers.

As for BDNF, there is an emerging body of data suggesting that different mood disorders are associated with changed BDNF. I think that changes of BDNF gene expression in the losers may be nonspecific for depression state. Expression of the BDNF gene in the winners should be investigated to confirm or reject this idea.

Again, Berton et al. (2006) have demonstrated very impressive data. Taking into consideration these data and our molecular studies, it may be suggested that the sensory contact paradigm (sensory contact model) may be used for the study of association between agonistic behavior and gene expression. We called this scientific direction “From behavior to gene” (reviewed in Kudryavtseva et al., 2004), as an addition to the traditional “From gene to behavior.”

References:

Kudryavtseva NN, Bakshtanovskaya IV, Koryakina LA. Social model of depression in mice of C57BL/6J strain. Pharmacol Biochem Behav. 1991 Feb;38(2):315-20. Abstract

Kudryavtseva NN, Avgustinovich DF. (1998) Behavioral and physiological markers of experimental depression induced by social conflicts (DISC). Aggress Behav. 24:271-286.

Filipenko ML, Alekseyenko OV, Beilina AG, Kamynina TP, Kudryavtseva NN. Increase of tyrosine hydroxylase and dopamine transporter mRNA levels in ventral tegmental area of male mice under influence of repeated aggression experience. Brain Res Mol Brain Res. 2001 Nov 30;96(1-2):77-81. Abstract

Filipenko ML, Beilina AG, Alekseyenko OV, Dolgov VV, Kudryavtseva NN. Repeated experience of social defeats increases serotonin transporter and monoamine oxidase A mRNA levels in raphe nuclei of male mice. Neurosci Lett. 2002 Mar 15;321(1-2):25-8. Abstract

Kudryavtseva et al. (2004) Changes in the expression of monoaminergic genes under the influence of repeated experience of agonistic interactions: From behavior to gene. Genetika, 40(6):732-748.

Avgustinovich DF, Alekseenko OV, Bakshtanovskaia IV, Koriakina LA, Lipina TV, Tenditnik MV, Bondar' NP, Kovalenko IL, Kudriavtseva NN. [Dynamic changes of brain serotonergic and dopaminergic activities during development of anxious depression: experimental study] Usp Fiziol Nauk. 2004 Oct-Dec;35(4):19-40. Review. Russian. Abstract

Goloshchapov AV, Filipenko ML, Bondar NP, Kudryavtseva NN, Van Ree JM. Decrease of kappa-opioid receptor mRNA level in ventral tegmental area of male mice after repeated experience of aggression. Brain Res Mol Brain Res. 2005 Apr 27;135(1-2):290-2. Epub 2005 Jan 6. Abstract

View all comments by NN Kudryavtseva